Serum Anti-Müllerian Hormone and Cytokine Profiling of Bubalus bubalis (Murrah buffalo) Calves for Puberty Prediction

S.H.  Sneha; Prahlad  Singh; Navdeep  Singh; Chanchal  Singh; Mrigank  Honparkhe

doi:10.6000/1927-520X.2024.13.07

Authors

S.H. Sneha Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India
Prahlad Singh Department of Teaching Veterinary Clinical Complex, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India https://orcid.org/0000-0001-5298-7781
Navdeep Singh Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India https://orcid.org/0000-0002-7095-4438
Chanchal Singh Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India
Mrigank Honparkhe Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana – 141004, Punjab, India

DOI:

https://doi.org/10.6000/1927-520X.2024.13.07

Keywords:

Cytokines, puberty, ELISA, buffalo, heifers, Karl Pearson correlation

Abstract

The present study incorporated ten buffalo calves aged 0 to 6 months, with an average weight of 35 kg, and ten buffalo heifers aged 12 months, with an average weight of 200 kg, to study the cytokine and AMH profile in relation to pubertal advancement. Venous blood samples (5ml) were collected from buffalo calves on the day of birth (day 0), day 15, day 30, day 60, day 90, day 120, day 150, and day 180. A single blood sample was collected from Buffalo heifers (1 year age). Cytokines: IFN-γ, IL-6, IL-1, IL-13, TNF-α, and TGF-β, and anti-Müllerian hormone: AMH were estimated using respective ELISA kits. At birth, cytokine levels in serum showed a varied pattern, with lower levels of IFN-γ, IL-6, and IL-13, whereas IL-1, TNF-α, and TGF-β were higher. Throughout the study, IFN-γ, IL-13, and TGF-β levels remained relatively stable, whereas IL-1, IL-6, and TNF-α increased notably by day 180. IL-1, TNF-α, and IL-6 levels were higher (P<0.01) from birth to 180 days as well as on day 365. AMH levels remained consistent from birth to 180 days, indicating a marked increase at Day 15 (33.49 ± 12.63 ng/L), followed by a decline to 4.60 ± 1.55 ng/Lat the end of the first year. Implications of the Karl Pearson correlation coefficient revealed a negative correlation between AMH levels and IFN-γ and TNF-α. AMH was positively correlated with IL 13 and TGF-β. Hence, it was concluded that IFN-γ and TNF-α are predictive markers for a reduction in AMH levels and hence, setting up puberty in buffalo heifers.

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