Prevalence of Bovine Immunodeficiency Virus Infection in Buffaloes in East Azerbaijan, Northwestern Iran

Authors

  • Mohammad Tolouei- Kaleibar Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
  • Morteza Mozaffari Large Animal Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Javad Ashrafi Veterinary Pathology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
  • Golamreza Nikbakht Veterinary Virology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  • Ezzatollah Fathi Veterinary Clinical Pathology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

DOI:

https://doi.org/10.6000/1927-520X.2018.07.02.3

Keywords:

Bovine immunodeficiency virus, PCR, Buffalo, Iran.

Abstract

Bovine immunodeficiency virus (BIV) has a worldwide distribution, but its prevalence in different regions of Iran is unknown. In this study, for the first time, the presence of BIV infections is detected by using the PCR method in Iranian water buffalo in East Azerbaijan. For this matter, blood samples were taken from 83 randomly selected buffaloes slaughtered in Tabriz industrial slaughterhouse from June to October 2012. All of the animals were clinically examined before sampling. Viral Gene-spin™ Viral DNA/RNA Extraction Kit was used to extract the DNA, and PCR was performed on the extracted DNA using oligonucleotiode primers specific for the gag gene region of the BIV virus. The prevalence of BIV in buffaloes was 2.4% (2 of 83), which is less than the prevalence of BIV in Pakistan (10.3%) and India (19%). The low prevalence observed in this study may be due to our small sample size.

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Published

2018-08-16

How to Cite

Kaleibar, M. T.-., Mozaffari, M., Ashrafi, J., Nikbakht, G., & Fathi, E. (2018). Prevalence of Bovine Immunodeficiency Virus Infection in Buffaloes in East Azerbaijan, Northwestern Iran. Journal of Buffalo Science, 7(2), 34–37. https://doi.org/10.6000/1927-520X.2018.07.02.3

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